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agar listeria  (Bio-Rad)


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    Bio-Rad agar listeria
    Agar Listeria, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agar+listeria/AL+(Agar+Listeria+according+to+Ottaviani+and+Agosti)+Agar+Plates/pm41901775-95-12-20
    Average 93 stars, based on 8 article reviews
    agar listeria - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Suspension:

    Article Title: Assessment of the Microbiological Quality of Raw Milk Sold Through Vending Machines at the Farm Level in Switzerland
    Article Snippet: The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. A total of 10 mL of the raw milk sample was enriched in 90 mL of buffered peptone water (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C overnight.

    Article Title: Occurrence and Characteristics of Salmonella spp., L. monocytogenes, Y. enterocolitica, and Campylobacter spp. in Raw Meat-Based Diets for Pets
    Article Snippet: Thereafter, 0.1 mL of the enrichment was transferred into 10 mL of Fraser broth (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated overnight at 37 ◦C. .. Next, one loopful of the suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated for 24–48 h at 37 ◦C. .. Suspicious blue colonies with a surrounding opaque halo were either identified at the species level using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS, Bruker Daltonics, Bremen, Germany), according to the manufacturer’s instructions (Bruker) by using the MBT Compass BDAL Library March 2023 from Bruker, or, initially, subcultured onto RAPID’ L. mono (Bio-Rad Laboratories AG, Cressier, Switzerland) plates for 24–48 h before further identification.

    Article Title: Microbiological characterization of randomly selected Portuguese raw milk cheeses with reference to food safety.
    Article Snippet: Cheese samples (25 g) were prepared in sterile buffered peptone water (225 ml; Merck, Darmstadt, Germany) and homogenized in a stomacher for 2 min. Ap- propriate decimal dilutions, to 10 4, were prepared in sterile Ringer’s solution (Merck) for the microbial enumeration of E. coli, coagulase-positive staphylococci, and L. monocytogenes. .. Decimal dilutions of the samples were inoculated on the following media. (i) Tryptone Bile X-glucuronide media were used (Bio-Rad, Hercules, Calif.) and incubated at 44 C for 24 h. Characteristic blue colonies of -glucuronidase–positive E. coli were enumerated according to the methodology of International Organization for Standardization (ISO) standard 16649-2 (6). (ii) Baird-Parker RPF agar was used (bioMérieux, Marcy l’Etoile, France) and incubated at 37 C for 48 h. Typical colonies (black or gray, small colonies surrounded by a halo of precipitation, indicating coagulase activity in this variation of Baird-Parker agar) of coagulase-positive staphylococci were enumerated according to ISO standard 6888-2 (5). (iii) Agar Listeria according to Ottaviani and Agosti (ALOA; BioRad) was used; in this case, only the initial suspension of the test sample (after revivification at 20 C for 1 h) and the 10 1 and 10 2 decimal dilutions were spread (0.1 ml) on ALOA plates, which were then incubated at 37 C for 48 h. Five green-blue colonies surrounded by an opaque halo on each counted plate were confirmed by morphological and biochemical tests, i.e., Gram staining, the catalase test, carbohydrate utilization (mannitol, rhamnose, and xylose), and the CAMP test. ..

    Incubation:

    Article Title: Assessment of the Microbiological Quality of Raw Milk Sold Through Vending Machines at the Farm Level in Switzerland
    Article Snippet: The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. A total of 10 mL of the raw milk sample was enriched in 90 mL of buffered peptone water (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C overnight.

    Article Title: Occurrence and Characteristics of Salmonella spp., L. monocytogenes, Y. enterocolitica, and Campylobacter spp. in Raw Meat-Based Diets for Pets
    Article Snippet: Thereafter, 0.1 mL of the enrichment was transferred into 10 mL of Fraser broth (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated overnight at 37 ◦C. .. Next, one loopful of the suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated for 24–48 h at 37 ◦C. .. Suspicious blue colonies with a surrounding opaque halo were either identified at the species level using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS, Bruker Daltonics, Bremen, Germany), according to the manufacturer’s instructions (Bruker) by using the MBT Compass BDAL Library March 2023 from Bruker, or, initially, subcultured onto RAPID’ L. mono (Bio-Rad Laboratories AG, Cressier, Switzerland) plates for 24–48 h before further identification.

    Article Title: Microbiological characterization of randomly selected Portuguese raw milk cheeses with reference to food safety.
    Article Snippet: Cheese samples (25 g) were prepared in sterile buffered peptone water (225 ml; Merck, Darmstadt, Germany) and homogenized in a stomacher for 2 min. Ap- propriate decimal dilutions, to 10 4, were prepared in sterile Ringer’s solution (Merck) for the microbial enumeration of E. coli, coagulase-positive staphylococci, and L. monocytogenes. .. Decimal dilutions of the samples were inoculated on the following media. (i) Tryptone Bile X-glucuronide media were used (Bio-Rad, Hercules, Calif.) and incubated at 44 C for 24 h. Characteristic blue colonies of -glucuronidase–positive E. coli were enumerated according to the methodology of International Organization for Standardization (ISO) standard 16649-2 (6). (ii) Baird-Parker RPF agar was used (bioMérieux, Marcy l’Etoile, France) and incubated at 37 C for 48 h. Typical colonies (black or gray, small colonies surrounded by a halo of precipitation, indicating coagulase activity in this variation of Baird-Parker agar) of coagulase-positive staphylococci were enumerated according to ISO standard 6888-2 (5). (iii) Agar Listeria according to Ottaviani and Agosti (ALOA; BioRad) was used; in this case, only the initial suspension of the test sample (after revivification at 20 C for 1 h) and the 10 1 and 10 2 decimal dilutions were spread (0.1 ml) on ALOA plates, which were then incubated at 37 C for 48 h. Five green-blue colonies surrounded by an opaque halo on each counted plate were confirmed by morphological and biochemical tests, i.e., Gram staining, the catalase test, carbohydrate utilization (mannitol, rhamnose, and xylose), and the CAMP test. ..

    Article Title: Listeria tempestatis sp. nov. and Listeria rocourtiae subsp. hofi subsp. nov.
    Article Snippet: .. Isolates were grown on Rapid’ L.mono agar (Bio-Rad) and Agar Listeria according to Ottaviani and Agosti (ALOA; bioMérieux) plates at 30 and 37 °C for 24 h. Motility was tested by stab inoculation in U-shaped glass tubes containing tryptic soy semi-solid agar (Bio-Rad), followed by incubation at 22 and 37 °C for 7 days in aerobic conditions. ..

    Article Title: Microbiological quality assessment of fresh produce: Potential health risk to children and urgent need for improved food safety in school feeding schemes
    Article Snippet: .. For the detection of L. monocytogenes and other Listeria species, 1 mL −1 of the overnight incubated sample in BPW was transferred to 9 mL −1 of buffered Listeria enrichment broth (Oxoid) and incubated at 37°C for 48 h and then streaked onto Agar Listeria according to Ottaviani and Agosti (BioRad, AEC Amersham, Johannesburg) and Rapid L. mono agar (BioRad). .. Salmonella spp. detection was done from samples incubated in BPW using the BioRad iQ check Salmonella kit (AEC Amersham), following the manufacturer's instructions (AOAC OMA 2017.06).

    Software:

    Article Title: Assessment of the Microbiological Quality of Raw Milk Sold Through Vending Machines at the Farm Level in Switzerland
    Article Snippet: The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. A total of 10 mL of the raw milk sample was enriched in 90 mL of buffered peptone water (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C overnight.

    Control:

    Article Title: Assessment of the Microbiological Quality of Raw Milk Sold Through Vending Machines at the Farm Level in Switzerland
    Article Snippet: The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. The next day, a loopful of the enriched suspension was inoculated onto Agar Listeria (AL) according to Ottaviani and Agosti (BioRad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C for 48 ± 2 h. Suspicious blue colonies with a surrounding opaque halo were identified by MALDI-TOF-MS, applying the software Flex Control 3.4., the MALDI Biotyper (MBT) Compass database version 4.1.100, and the MBT Compass BDAL 12.0 Library. .. A total of 10 mL of the raw milk sample was enriched in 90 mL of buffered peptone water (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated at 37 °C overnight.

    Activity Assay:

    Article Title: Microbiological characterization of randomly selected Portuguese raw milk cheeses with reference to food safety.
    Article Snippet: Cheese samples (25 g) were prepared in sterile buffered peptone water (225 ml; Merck, Darmstadt, Germany) and homogenized in a stomacher for 2 min. Ap- propriate decimal dilutions, to 10 4, were prepared in sterile Ringer’s solution (Merck) for the microbial enumeration of E. coli, coagulase-positive staphylococci, and L. monocytogenes. .. Decimal dilutions of the samples were inoculated on the following media. (i) Tryptone Bile X-glucuronide media were used (Bio-Rad, Hercules, Calif.) and incubated at 44 C for 24 h. Characteristic blue colonies of -glucuronidase–positive E. coli were enumerated according to the methodology of International Organization for Standardization (ISO) standard 16649-2 (6). (ii) Baird-Parker RPF agar was used (bioMérieux, Marcy l’Etoile, France) and incubated at 37 C for 48 h. Typical colonies (black or gray, small colonies surrounded by a halo of precipitation, indicating coagulase activity in this variation of Baird-Parker agar) of coagulase-positive staphylococci were enumerated according to ISO standard 6888-2 (5). (iii) Agar Listeria according to Ottaviani and Agosti (ALOA; BioRad) was used; in this case, only the initial suspension of the test sample (after revivification at 20 C for 1 h) and the 10 1 and 10 2 decimal dilutions were spread (0.1 ml) on ALOA plates, which were then incubated at 37 C for 48 h. Five green-blue colonies surrounded by an opaque halo on each counted plate were confirmed by morphological and biochemical tests, i.e., Gram staining, the catalase test, carbohydrate utilization (mannitol, rhamnose, and xylose), and the CAMP test. ..

    Staining:

    Article Title: Microbiological characterization of randomly selected Portuguese raw milk cheeses with reference to food safety.
    Article Snippet: Cheese samples (25 g) were prepared in sterile buffered peptone water (225 ml; Merck, Darmstadt, Germany) and homogenized in a stomacher for 2 min. Ap- propriate decimal dilutions, to 10 4, were prepared in sterile Ringer’s solution (Merck) for the microbial enumeration of E. coli, coagulase-positive staphylococci, and L. monocytogenes. .. Decimal dilutions of the samples were inoculated on the following media. (i) Tryptone Bile X-glucuronide media were used (Bio-Rad, Hercules, Calif.) and incubated at 44 C for 24 h. Characteristic blue colonies of -glucuronidase–positive E. coli were enumerated according to the methodology of International Organization for Standardization (ISO) standard 16649-2 (6). (ii) Baird-Parker RPF agar was used (bioMérieux, Marcy l’Etoile, France) and incubated at 37 C for 48 h. Typical colonies (black or gray, small colonies surrounded by a halo of precipitation, indicating coagulase activity in this variation of Baird-Parker agar) of coagulase-positive staphylococci were enumerated according to ISO standard 6888-2 (5). (iii) Agar Listeria according to Ottaviani and Agosti (ALOA; BioRad) was used; in this case, only the initial suspension of the test sample (after revivification at 20 C for 1 h) and the 10 1 and 10 2 decimal dilutions were spread (0.1 ml) on ALOA plates, which were then incubated at 37 C for 48 h. Five green-blue colonies surrounded by an opaque halo on each counted plate were confirmed by morphological and biochemical tests, i.e., Gram staining, the catalase test, carbohydrate utilization (mannitol, rhamnose, and xylose), and the CAMP test. ..

    other:

    Article Title: Assessment of the Microbiological Quality of Raw Milk Sold Through Vending Machines at the Farm Level in Switzerland.
    Article Snippet: A total of 10 mL of the raw milk sample was enriched in 90 mL of buffered peptone water (Bio-Rad Laboratories AG, Cressier, Switzerland) and incubated at 37 ◦C overnight.

    Glass:

    Article Title: Listeria tempestatis sp. nov. and Listeria rocourtiae subsp. hofi subsp. nov.
    Article Snippet: .. Isolates were grown on Rapid’ L.mono agar (Bio-Rad) and Agar Listeria according to Ottaviani and Agosti (ALOA; bioMérieux) plates at 30 and 37 °C for 24 h. Motility was tested by stab inoculation in U-shaped glass tubes containing tryptic soy semi-solid agar (Bio-Rad), followed by incubation at 22 and 37 °C for 7 days in aerobic conditions. ..

    Pulsed-Field Gel:

    Article Title: Occurrence of Foodborne Agents at Food Service Facilities in the Czech Republic
    Article Snippet: The aim of this study was to investigate the occurrence of foodborne agents at food service facilities in the Czech Republic.. The sampling, performed from April 2016 to November 2017, focused on the microbiological monitoring of the environment at the establishment (EFS; n 1⁄4 298) and the hands of staff (HFS; n 1⁄4 159).. The analysis targeted the presence of the following bacteria: Escherichia coli (focusing on the presence of Shiga toxigenic E. coli), Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella spp., and Campylobacter spp. .. The aim of this study was to investigate the occurrence of foodborne agents at food service facilities in the Czech Republic.. The sampling, performed from April 2016 to November 2017, focused on the microbiological monitoring of the environment at the establishment (EFS; n 1⁄4 298) and the hands of staff (HFS; n 1⁄4 159).. The analysis targeted the presence of the following bacteria: Escherichia coli (focusing on the presence of Shiga toxigenic E. coli), Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella spp., and Campylobacter spp. ..



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